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rnawas extractedwith trizol  (TaKaRa)


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    Structured Review

    TaKaRa rnawas extractedwith trizol
    Rnawas Extractedwith Trizol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trizol+kit/PrimeScript+RT+Reagent+Kit/pm42864323-371-0-4
    Average 99 stars, based on 89544 article reviews
    rnawas extractedwith trizol - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The E3 ubiquitin ligase NEDD4 protects against nonesterified fatty acid-induced hepatic inflammatory injury in ketotic cows.
    Article Snippet: .. For the quantitative real-time PCR (qRT-PCR) experiment, total RNA was extracted from calf primary hepatocytes using the TRIzol kit (9109, Takara Biotechnology Co. Ltd.) in accordance with the manufacturer's instructions. .. The RNA concentration was measured using the Gene Quant II RNA/DNA calculator (Amersham Pharmacia Biotech).

    Article Title: Extracts of Chuanxiong and Baizhi Attenuate Neuroinflammation in Chronic Migraine Rats by Inhibiting TLR4/MyD88/Nuclear Factor Kappa B Signal Pathway
    Article Snippet: The cell apoptosis was determined using FACSCanto II flow cytometry (BD Biosciences, USA) and Flowjo soft (TreeStar, USA). .. 3Journal of Physiological Investigation ¦ Volume XX ¦ Issue XX ¦ Month 2025 RNA isolation and quantitative real‐time polymerase chain reaction Total RNA was extracted from the cells using Trizol kit (Takara, Dalian, China) to obtain cDNA through reverse transcription. .. Polymerase chain reaction (PCR) was performed using SYBR Prime Script real‐time‐PCR Kit (Takara Bio, Inc.) with the reaction volume of 10 μl which contains cDNA 0.8 μl, SYBR Primix Ex Taq 5.0 μl, primer 1.0 μl and RNase H2O 2.2 μl.

    Polymerase Chain Reaction:

    Article Title: Mulberroside A mitigates intervertebral disc degeneration by inhibiting MAPK and modulating Ppar-γ/NF-κB pathways.
    Article Snippet: Bovine serum albumin (5%) was used to block the protein bands, which were then incubated with corresponding primary antibodies listed in Table 1 at 4 °C for 16 h, and with secondary antibodies at room temperature for 1 h. Finally, Image Lab System (Bio-Rad, USA) was used to analyse the target protein bands. .. Real time-quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted using a TRIZOL kit (Takara Bio Inc, Japan), and cDNA was synthesized using a cDNA synthesis kit (Yeasen, China). ..

    Article Title: Hedgehog/Gli2 signaling triggers cell proliferation and metastasis via EMT and wnt/β-catenin pathways in oral squamous cell carcinoma
    Article Snippet: .. Total RNA was extracted by using a TRIzol kit (Takara, Japan), and cDNA was synthesized using a reverse transcription kit (Takara, Japan). qRT-PCR was performed on an Applied Biosystems 7500 (ABI 7500) system (Thermofisher, USA) using a SYBR Green PCR Kit (Takara, Japan). ..

    Synthesized:

    Article Title: Mulberroside A mitigates intervertebral disc degeneration by inhibiting MAPK and modulating Ppar-γ/NF-κB pathways.
    Article Snippet: Bovine serum albumin (5%) was used to block the protein bands, which were then incubated with corresponding primary antibodies listed in Table 1 at 4 °C for 16 h, and with secondary antibodies at room temperature for 1 h. Finally, Image Lab System (Bio-Rad, USA) was used to analyse the target protein bands. .. Real time-quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted using a TRIZOL kit (Takara Bio Inc, Japan), and cDNA was synthesized using a cDNA synthesis kit (Yeasen, China). ..

    Article Title: Hedgehog/Gli2 signaling triggers cell proliferation and metastasis via EMT and wnt/β-catenin pathways in oral squamous cell carcinoma
    Article Snippet: .. Total RNA was extracted by using a TRIzol kit (Takara, Japan), and cDNA was synthesized using a reverse transcription kit (Takara, Japan). qRT-PCR was performed on an Applied Biosystems 7500 (ABI 7500) system (Thermofisher, USA) using a SYBR Green PCR Kit (Takara, Japan). ..

    cDNA Synthesis:

    Article Title: Mulberroside A mitigates intervertebral disc degeneration by inhibiting MAPK and modulating Ppar-γ/NF-κB pathways.
    Article Snippet: Bovine serum albumin (5%) was used to block the protein bands, which were then incubated with corresponding primary antibodies listed in Table 1 at 4 °C for 16 h, and with secondary antibodies at room temperature for 1 h. Finally, Image Lab System (Bio-Rad, USA) was used to analyse the target protein bands. .. Real time-quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted using a TRIZOL kit (Takara Bio Inc, Japan), and cDNA was synthesized using a cDNA synthesis kit (Yeasen, China). ..

    Isolation:

    Article Title: Comprehensive Analysis of lncRNA/circRNAs-miRNA-mRNA Networks of Oral Lichen Planus
    Article Snippet: .. Total RNA of each sample was isolated using TRIZOL kit (TaKaRa, 9109). ..

    Article Title: Comprehensive Analysis of lncRNA/circRNAs-miRNA-mRNA Networks of Oral Lichen Planus
    Article Snippet: .. Total RNA of each sample was isolated using TRIZOL kit (TaKaRa, 9109). ..

    Article Title: Extracts of Chuanxiong and Baizhi Attenuate Neuroinflammation in Chronic Migraine Rats by Inhibiting TLR4/MyD88/Nuclear Factor Kappa B Signal Pathway
    Article Snippet: The cell apoptosis was determined using FACSCanto II flow cytometry (BD Biosciences, USA) and Flowjo soft (TreeStar, USA). .. 3Journal of Physiological Investigation ¦ Volume XX ¦ Issue XX ¦ Month 2025 RNA isolation and quantitative real‐time polymerase chain reaction Total RNA was extracted from the cells using Trizol kit (Takara, Dalian, China) to obtain cDNA through reverse transcription. .. Polymerase chain reaction (PCR) was performed using SYBR Prime Script real‐time‐PCR Kit (Takara Bio, Inc.) with the reaction volume of 10 μl which contains cDNA 0.8 μl, SYBR Primix Ex Taq 5.0 μl, primer 1.0 μl and RNase H2O 2.2 μl.

    Reverse Transcription:

    Article Title: Downregulation of HSPB1 and MGST1 Promotes Ferroptosis and Impacts Immune Infiltration in Diabetic Cardiomyopathy.
    Article Snippet: Diabetic cardiomyopathy (DCM) is a leading cause of death in diabetic patients.. Current therapies do not adequately resolve this problem and focus only on the optimal level of blood glucose for patients.. Ferroptosis plays an important role in diabetes mellitus and cardiovascular diseases.

    Article Title: Hedgehog/Gli2 signaling triggers cell proliferation and metastasis via EMT and wnt/β-catenin pathways in oral squamous cell carcinoma
    Article Snippet: .. Total RNA was extracted by using a TRIzol kit (Takara, Japan), and cDNA was synthesized using a reverse transcription kit (Takara, Japan). qRT-PCR was performed on an Applied Biosystems 7500 (ABI 7500) system (Thermofisher, USA) using a SYBR Green PCR Kit (Takara, Japan). ..

    Article Title: Extracts of Chuanxiong and Baizhi Attenuate Neuroinflammation in Chronic Migraine Rats by Inhibiting TLR4/MyD88/Nuclear Factor Kappa B Signal Pathway
    Article Snippet: The cell apoptosis was determined using FACSCanto II flow cytometry (BD Biosciences, USA) and Flowjo soft (TreeStar, USA). .. 3Journal of Physiological Investigation ¦ Volume XX ¦ Issue XX ¦ Month 2025 RNA isolation and quantitative real‐time polymerase chain reaction Total RNA was extracted from the cells using Trizol kit (Takara, Dalian, China) to obtain cDNA through reverse transcription. .. Polymerase chain reaction (PCR) was performed using SYBR Prime Script real‐time‐PCR Kit (Takara Bio, Inc.) with the reaction volume of 10 μl which contains cDNA 0.8 μl, SYBR Primix Ex Taq 5.0 μl, primer 1.0 μl and RNase H2O 2.2 μl.

    Quantitative RT-PCR:

    Article Title: Hedgehog/Gli2 signaling triggers cell proliferation and metastasis via EMT and wnt/β-catenin pathways in oral squamous cell carcinoma
    Article Snippet: .. Total RNA was extracted by using a TRIzol kit (Takara, Japan), and cDNA was synthesized using a reverse transcription kit (Takara, Japan). qRT-PCR was performed on an Applied Biosystems 7500 (ABI 7500) system (Thermofisher, USA) using a SYBR Green PCR Kit (Takara, Japan). ..

    SYBR Green Assay:

    Article Title: Hedgehog/Gli2 signaling triggers cell proliferation and metastasis via EMT and wnt/β-catenin pathways in oral squamous cell carcinoma
    Article Snippet: .. Total RNA was extracted by using a TRIzol kit (Takara, Japan), and cDNA was synthesized using a reverse transcription kit (Takara, Japan). qRT-PCR was performed on an Applied Biosystems 7500 (ABI 7500) system (Thermofisher, USA) using a SYBR Green PCR Kit (Takara, Japan). ..



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